(A) Representative plots showing mean percentages of CD4⁺ and CD8⁺ T cells, respectively, and percentage CXCR3 positivity of CD4⁺ T cells, as well as relative chemokine receptor expression of CD3⁺CD4⁺ T cells obtained from nephritic animals at day 10 of cGN determined by flow cytometric analyses. (B) Analysis of the single-cell RNA-Seq data of renal CD3⁺ T cells of nephritic mice analyzed for relative chemokine receptor expression of the CD4_naive and CD4_memory clusters. (C and D) Schematic representation of the experimental setup, and quantification of flow cytometric analyses of absolute numbers of CD3⁺CD4⁺IFN-γ⁺ T cells isolated from kidneys of untreated and steroid-treated nephritic mice. (E) Reverse transcription PCR (RT-PCR) analyses of indicated mRNA expression from whole renal cortices of kidneys collected from the groups mentioned before. (F) RNA scope analyses show the preferential localization of Cxcl9 and Cxcl10 mRNA to the tubulointerstitial compartment and markedly reduced expression in steroid treated mice. Scale of images is indicated by 1 cm in width equaling 15 µm. (G) Heatmap of chemokine protein levels in supernatants of proximal tubular epithelial cells after stimulation with either medium alone, IL-17A, IFN-γ, or TNF-α under increasing concentrations of prednisolone. (H and I) Schematic representation of the experimental setup, and numbers of recovered renal CD4⁺ T cells from PBS-treated and steroid- pulsed nephritic Rag1^–/– mice after eleven days following i.v. transfer of CXCR3^–CD4⁺ T cells into both groups. (J) Semiquantitative analysis of intrarenal T cell numbers derived from kidney sections IHC stained for CD3 for the groups mentioned before. Symbols represent individual data points, with the mean as a bar graph. Data were analyzed using a 2-tailed t test. *P < 0.05, **P < 0.01.