(A) Venn diagram showing 2 overlapping genes between the differently expressed genes detected by RNA-Seq and the potential target genes of RFX1 determined by ChIP-Seq. (B) Volcano plot of differentially expressed genes between control and RFX1-overexpressed M1 hMDMs (n = 3). (C) The ChIP-Seq profile of RFX1 in the APOBEC3A gene locus and sequence logo of the RFX1 motif were indicated. (D) The relative expression of luciferase in each group was determined by a luciferase reporter assay containing the genomic fragment of APOBEC3A in 293T cells (n = 5). (E) The ChIP-qPCR was performed on 293T cells infected with pLV-RFX1 using anti-FLAG antibodies (n = 3). (F and G) The relative mRNA (n = 5) (F) and protein (n = 3) (G) expressions of Apobec3 in PMAs were analyzed. The protein expression was shown after being normalized β-tublin. (H and I) The relative mRNA (n = 6 per group) (H) and protein (n = 2 per group) (I) expressions of Apobec3 in M1 PMAs from WT and CKO mice were determined. (J and K) Western blot was used to determine the protein expressions of Apobec3 in PMAs with indicated treatment (J, n = 4; K, n = 3). The protein expressions were shown after being normalized to GAPDH. (L and M) The mRNA (L) and protein (M) expressions of Il6, Il1b, and Tnf in PMAs infected with pLV-NC and pLV-Apobec3 (n = 3). (N) The mRNA expressions of Arg1, Chils, and Mrc1 in PMAs infected with pLV-NC and pLV-Apobec3 (n = 3). Data represent mean ± SEM. One-way ANOVA with Dunnett’s multiple-comparison test was used in J and K. Two-tailed Student’s t test was used in D–H and L–N. *P < 0.05, **P < 0.01, ***P < 0.001.