(A) Schematic of locomotor activity recording before and through GBM progression. We recorded wheel-running activity continuously from mice for 7 days before and continuously through disease progression after GBM implant into the basal ganglia. (B) Daily locomotor activity of mice bearing patient-derived B165 tumors significantly declined 24 days after implant (mean ± SEM, n reported in figure, asterisk indicates day of 10% locomotion decline, time series change point detection analysis, *P < 0.05). Dotted lines on days 9–12 indicate dates of surgical recovery when locomotor activity was not recorded. (C) Daily locomotor activity of mice bearing syngeneic murine Nf1^–/–DNp53 tumors significantly declined 37 days after implant (mean ± SEM, n reported in figure, asterisk indicates day of 10% locomotion decline, time series change point detection analysis, *P < 0.05). Dotted lines on days 9–12 indicate dates of surgical recovery when locomotor activity was not recorded. (D) Daily locomotor activity of mice bearing human LN229 tumors significantly declined 17 days after implant (mean ± SEM, n reported in figure, asterisk indicates day of 10% locomotion decline, time series change point detection analysis, *P < 0.05). Dotted lines on days 9–12 indicate dates of surgical recovery when locomotor activity was not recorded. (E) Daily locomotor activity of mice bearing murine GL261 tumors significantly declined 15 days after implant (mean ± SEM, n reported in figure, asterisk indicates day of 10% locomotion decline, time series change point detection analysis, *P < 0.05). Dotted lines on days 9–12 indicate dates of surgical recovery when locomotor activity was not recorded.